abstract：:A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of Cucurbit chlorotic yellows virus (CCYV). In this procedure, a set of four primers matching a total of six sequences in the coat protein gene region of CCYV was synthesized for the RT-LAMP assay using total...

journal_title：Journal of virological methods

authors： Wang Z,Gu Q,Sun H,Li H,Sun B,Liang X,Yuan Y,Liu R,Shi Y

更新日期：2014-01-01 00:00:00

abstract：:Although hepatitis C virus (HCV) is a pathogen of global significance, experimental therapies in current clinical development include highly efficacious all-oral combinations of HCV direct-acting antivirals (DAAs). If approved for use, these new treatment regimens will impact dramatically upon our capacity to eradicat...

journal_title：Journal of virological methods

authors： Kylefjord H,Danielsson A,Sedig S,Belda O,Wiktelius D,Vrang L,Targett-Adams P

更新日期：2014-01-01 00:00:00

abstract：:Lujo virus is an emerging arenavirus circulating in Southern Africa. Although to date there has only been a single outbreak of the novel haemorrhagic disease resulting from human infection with this virus, the case-fatality rate of exposed individuals, including nosocomial transmission, was 80%. The ability to identif...

journal_title：Journal of virological methods

authors： Atkinson B,Chamberlain J,Dowall SD,Cook N,Bruce C,Hewson R

更新日期：2014-01-01 00:00:00

abstract：:The hemagglutinin (HA) of avian influenza viruses plays a very important role in the infection of host cells. In this study, the HA gene of the highly pathogenic avian influenza H5N1 virus was cloned and expressed in silkworm larvae. The expressed recombinant HA (rHA) was purified using fetuin-agarose chromatography a...

journal_title：Journal of virological methods

authors： Dong J,Harada M,Yoshida S,Kato Y,Murakawa A,Ogata M,Kato T,Usui T,Park EY

更新日期：2013-12-01 00:00:00

abstract：:Frequent outbreaks of grass carp hemorrhagic disease, caused by grass carp reovirus (GCRV) infection, pose as serious threats to the production of grass carp Ctenopharyngodon idella. Although various nucleic acids-based diagnostic methods have been shown effective, lack of commercial monoclonal antibody against grass ...

journal_title：Journal of virological methods

authors： He Y,Jiang Y,Lu L

更新日期：2013-12-01 00:00:00

abstract：:A full glycoprotein E (gE) deletion was generated in genome of the Egyptian BoHV-1.1 Abu-Hammad strain. Integrity of the gE negative (gE(-)) mutant virus was proved by successful specific PCR amplifications of gB, gC, tk, gD, gI and gE genes along with definite immune reaction to polyclonal anti-BoHV-1 antibody in inf...

journal_title：Journal of virological methods

authors： El-Kholy AA,Rady DI,Abdou ER,Elseafy MM,Abdelrahman KA,Soliman H

更新日期：2013-12-01 00:00:00

abstract：:A novel reassortant influenza A (H7N9) virus emerged recently in China. In this study, a duplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay was developed for the simultaneous detection of hemagglutinin (HA) and neuraminidase (NA) genes of H7N9 influenza viruses. The sensitivity of the ass...

journal_title：Journal of virological methods

authors： Li Y,Wu T,Qi X,Ge Y,Guo X,Wu B,Yu H,Zhu Y,Shi Z,Wang H,Cui L,Zhou M

更新日期：2013-12-01 00:00:00

abstract：:Heteroduplex mobility (HMA) and tracking assays (HTA) are used to assess genetic relationships between DNA molecules. While distinguishing relationships between clonal or nearly clonal molecules is relatively straightforward, inferring population structures is more complex. To address this issue, HIV-1 quasispecies wi...

journal_title：Journal of virological methods

authors： Shankarappa R,Mullins JI

更新日期：2013-12-01 00:00:00

abstract：:HIV-1 RNA viral load is the preferred tool to monitor virological failure during antiretroviral therapy (ART) exposure. Timely detection of virological failure can reduce the prevalence and complexity of HIV-1 drug resistance. This field evaluation further characterizes a two-step approach to identify virological fail...

journal_title：Journal of virological methods

authors： Bronze M,Aitken SC,Wallis CL,Steegen K,Stuyver LJ,de Wit TF,Stevens W

更新日期：2013-12-01 00:00:00

abstract：:Measuring the in vitro replication capacity of viruses is an important tool for assessing the effects of selective pressure of immune responses and drug therapy. Measuring hepatitis C virus (HCV) replication capacity utilizing primarily sub-genomic reporter constructs is limited. To overcome some of these limitations ...

journal_title：Journal of virological methods

authors： Lassmann B,Arumugaswami V,Chew KW,Lewis MJ

更新日期：2013-12-01 00:00:00

abstract：:Sendai virus (SeV) is strictly monitored in laboratory rodents. Currently, complete virions have been used as antigens in SeV serological tests. However, the complexity of SeV virion antigen limits the accuracy of the diagnostic method. In the current study, complete SeV virion antigen was separated on SDS-PAGE and an...

journal_title：Journal of virological methods

authors： Xiang Z,Tong W,Li Y,Qin C,Wei Q

更新日期：2013-11-01 00:00:00

abstract：:Rice stripe virus (RSV), Rice black-streaked dwarf virus (RBSDV), and Rice dwarf virus (RDV) are major rice-infecting viruses in Korea that can cause serious crop losses. A one-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for the simultaneous detection of these rice viruses. T...

journal_title：Journal of virological methods

authors： Cho SY,Jeong RD,Yoon YN,Lee SH,Shin DB,Kang HW,Lee BC

更新日期：2013-11-01 00:00:00

abstract：:Hepatitis D virus (HDV) is a defective virus which requires hepatitis B virus (HBV) surface antigen (HBsAg) for its assembly. Hepatitis B infected individuals co-infected or superinfected with HDV often present with more severe hepatitis, progress faster to liver disease, and have a higher mortality rate than individu...

journal_title：Journal of virological methods

authors： Kodani M,Martin A,Mixson-Hayden T,Drobeniuc J,Gish RR,Kamili S

更新日期：2013-11-01 00:00:00

abstract：:Virus titration may constitute a drawback in the development and use of replication-defective viral vectors like Semliki Forest virus (SFV). The standardization and validation of a reverse transcription quantitative PCR (qRT-PCR) method for SFV titration is presented here. The qRT-PCR target is located within the nsp1...

journal_title：Journal of virological methods

authors： Puglia AL,Rezende AG,Jorge SA,Wagner R,Pereira CA,Astray RM

更新日期：2013-11-01 00:00:00

abstract：:Contaminated recreational waters pose a public health concern, as the potential for waterborne diseases exists in water contaminated with human fecal waste. Worldwide, bacterial indicators such as Escherichia coli, enterococci, and total and fecal coliform are used as indicators of water quality. However, enteric viru...

journal_title：Journal of virological methods

authors： Allmann E,Pan L,Li L,Li D,Wang S,Lu Y

更新日期：2013-11-01 00:00:00

abstract：:Monitoring the serum hepatitis B viral DNA levels with sensitive realtime PCR assays is strongly recommended for the management of patients with chronic HBV infection. This study compares the performance of two realtime HBV quantitative PCR assays with samples from chronic HBeAg(+) and HBeAg(-) patients. ...

journal_title：Journal of virological methods

authors： Morris CJ,Hill M,de Medina M,Herman C,Cloherty GA,Martin P

更新日期：2013-11-01 00:00:00

abstract：:HSV-1-based vectors have been widely used to achieve targeted delivery of genes into the nervous system. In the current study, we aim to use shRNA-containing HSV-1-based gene delivery system for the therapy of HSV-2 infection. Guinea pigs were infected intravaginally with HSV-2 and scored daily for 100 days for the se...

journal_title：Journal of virological methods

authors： Liu Z,Xiang Y,Wei Z,Yu B,Shao Y,Zhang J,Yang H,Li M,Guan M,Wan J,Zhang W

更新日期：2013-11-01 00:00:00

abstract：:PRRSV is a positive-sense RNA virus with a high degree of genetic variability among isolates. For diagnostic sensitivity and vaccine design it is essential to monitor PRRSV genetic diversity. However, to date only a few full genome sequences of PRRSV isolates have been made publicly available. In the present study, fa...

journal_title：Journal of virological methods

authors： Kvisgaard LK,Hjulsager CK,Fahnøe U,Breum SØ,Ait-Ali T,Larsen LE

更新日期：2013-11-01 00:00:00

abstract：:A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N=75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa=0.57 95% CI 0.3-0.89). Positive percent agreement was 92.3% (60...

journal_title：Journal of virological methods

authors： A Murillo L,Hardick J,Jeng K,Gaydos CA

更新日期：2013-10-01 00:00:00

abstract：:Genotypic drug resistance testing is routine practice in HIV-1 clinical care. The visual interpretation of sequencing electropherograms is labour-intensive and subject to intra- and inter-assay variability because decisions are based on operators' judgments. In this study the performance of the automatic editing tool ...

journal_title：Journal of virological methods

authors： Vinken L,Megens S,Schrooten Y,Vandamme AM,Van Laethem K

更新日期：2013-10-01 00:00:00

abstract：:Paramyxoviruses, including measles virus (MV), human metapneumovirus (HMPV), human respiratory syncytial virus (HRSV) and canine distemper virus (CDV), are transmitted via the respiratory route. Despite their close phylogenetic relationship, the pathogenesis of these viruses is very different. To study viral tropism a...

journal_title：Journal of virological methods

authors： Nguyen DT,de Vries RD,Ludlow M,van den Hoogen BG,Lemon K,van Amerongen G,Osterhaus AD,de Swart RL,Duprex WP

更新日期：2013-10-01 00:00:00

abstract：:The prevalence of porcine teschovirus (PTV) in swine herds in northeast China was investigated. In 2008-2009, 1384 samples of pig sera were collected from 42 farms in Shandong, Hebei, Heilongjiang, and Jilin provinces and in Tianjin City and were tested for specific antibody against PTV-8 by immunofluorescence assay. ...

journal_title：Journal of virological methods

authors： Qiu Z,Wang Z,Zhang B,Zhang J,Cui S

更新日期：2013-10-01 00:00:00

abstract：:Squash leaf curl diseases are caused by distinct virus species that are separated into two major phylogenetic groups, western and eastern hemisphere groups. The western group includes the new world Squash leaf curl virus (SLCV) which causes major losses to cucurbit production and induces severe stunting and leaf curl ...

journal_title：Journal of virological methods

authors： Abrahamian PE,Abou-Jawdah Y

更新日期：2013-07-01 00:00:00

abstract：:To develop a quantitative assay for universal detection of duck hepatitis B virus (DHBV) DNA, a Taqman real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) assay was developed using primers and probes based on genomic sequences located at nucleotide 241-414 of the DHBV Core region which possesses the ...

journal_title：Journal of virological methods

authors： Wang Y,Li Y,Yang C,Hui L,Han Q,Ma L,Wang Q,Yang G,Liu Z

更新日期：2013-07-01 00:00:00

abstract：:Impatiens necrotic spot virus (INSV) is an important plant virus that can cause severe disease in various ornamental and agricultural crops. Several species of thrips transmit INSV, of which the western flower thrip (Frankliniella occidentalis) is the most important. In this study, primers and TaqMan probes based on I...

journal_title：Journal of virological methods

authors： Chen X,Xu X,Li Y,Liu Y

更新日期：2013-05-01 00:00:00

abstract：:Bacterial ghosts offer a new avenue for the study of inactivated vaccines. However, for many years the mechanism of genetic inactivation was controversial. To obtain mouse monoclonal antibodies (mAbs) against protein E will allow the observation of its location and dynamic expression to expand understanding of the lys...

journal_title：Journal of virological methods

authors： Yu S,Zhao H,Wang H,Wang X,Shao G,Xu L,Si W,Chen L,Zhang W,Liu S

更新日期：2013-05-01 00:00:00

abstract：:Lymphocystis disease virus (LCDV) is the causative agent of lymphocystis disease affecting marine and freshwater fish worldwide. Here an antibody microarray was developed and employed to detect LCDV in fish. Rabbit anti-LCDV serum was arrayed on agarose gel-modified slides as capture antibody, and Cy3-conjugated anti-...

journal_title：Journal of virological methods

authors： Sheng X,Xu X,Zhan W

更新日期：2013-05-01 00:00:00

abstract：:Lentiviral vectors (LVs) are promising delivery systems for gene therapy. To enhance the efficiency of target cell transduction by LVs, protocols often include the addition of culture additives. In this study, the cationic amphipathic peptide LAH4-L1 (KKALLAHALHLLALLALHLAHALKKA), a DNA transfection agent, was evaluate...

journal_title：Journal of virological methods

authors： Fenard D,Genries S,Scherman D,Galy A,Martin S,Kichler A

更新日期：2013-05-01 00:00:00

abstract：:In the context of eradication of poliomyelitis the World Health Organization stimulates the development of inactivated polio vaccines based on attenuated virus strains. In addition to vaccine development, tests have to be designed to assess the vaccine quality. An important test is the identification test for poliovir...

journal_title：Journal of virological methods

authors： Nijst OE,Mouthaan JJ,Mekkes DR,Jusic E,van der Avoort HG,Metz B

更新日期：2013-04-01 00:00:00

abstract：:In the current study, primers described previously and modified versions of these primers were evaluated for amplification of full-length gag genes from different equine infectious anemia virus (EIAV) strains from several countries, including the USA, Germany and Japan. Each strain was inoculated into a primary horse ...

journal_title：Journal of virological methods

authors： Boldbaatar B,Bazartseren T,Koba R,Murakami H,Oguma K,Murakami K,Sentsui H

更新日期：2013-04-01 00:00:00

abstract：:A single-tube duplex and multiplex PCR was developed for the simultaneous detection of African cassava mosaic virus (ACMV), East African cassava mosaic Cameroon virus (EACMCV), East African cassava mosaic Malawi virus (EACMMV) and East African cassava mosaic Zanzibar virus (EACMZV), four cassava mosaic begomoviruses (...

journal_title：Journal of virological methods

authors： Aloyce RC,Tairo F,Sseruwagi P,Rey ME,Ndunguru J

更新日期：2013-04-01 00:00:00

abstract：:Expression of recombinant beak and feather disease virus (BFDV) capsid-associated protein (Cap) has relied on inefficient techniques that typically produce low yields or use specialized expression systems, which greatly increase the cost and expertise required for mass production. An Escherichia coli system was used t...

journal_title：Journal of virological methods

authors： Patterson EI,Swarbrick CM,Roman N,Forwood JK,Raidal SR

更新日期：2013-04-01 00:00:00

abstract：:Foot-and-mouth disease (FMD) is a highly infectious viral disease of cloven-hoofed animals with debilitating and devastating consequences for livestock industries throughout the world. Key antigenic determinants of the causative agent, FMD virus (FMDV), reside within the surface-exposed proteins of the viral capsid. T...

journal_title：Journal of virological methods

authors： Xu L,Hurtle W,Rowland JM,Casteran KA,Bucko SM,Grau FR,Valdazo-González B,Knowles NJ,King DP,Beckham TR,McIntosh MT

更新日期：2013-04-01 00:00:00

abstract：:Rapid alternative methods are required to evaluate easily acyclovir (ACV) sensitivity of clinical herpes simplex virus (HSV) isolates. The objective of this study was to screen 54 ACV-sensitive and 41 ACV-resistant clinical HSV-1 isolates, well characterized by phenotypic and genotypic methods, for the phosphorylation...

journal_title：Journal of virological methods

authors： Sauerbrei A,Vödisch S,Bohn K,Schacke M,Gronowitz S

更新日期：2013-03-01 00:00:00

abstract：:Nucleic acid amplification techniques have become the mainstay for ultimate sensitivity for detecting low levels of virus, including human immunodeficiency virus (HIV). As a sophisticated technology with relative expensive reagents and instrumentation, adoption of nucleic acid testing (NAT) can be cost inhibited in se...

journal_title：Journal of virological methods

authors： Chang L,Song L,Fournier DR,Kan CW,Patel PP,Ferrell EP,Pink BA,Minnehan KA,Hanlon DW,Duffy DC,Wilson DH

更新日期：2013-03-01 00:00:00

abstract：:Most transplant centers screen kidney transplant recipients for BK virus (BKV) infection using molecular techniques for the virus load determination. However, there is no consensus about the pre-analytical methods involved in the viral detection. In this study BK viral load was compared by the means of two urine treat...

journal_title：Journal of virological methods

authors： Pinto GG,Poloni JA,Carneiro LC,Baethgen LF,Barth AL,Pasqualotto AC

更新日期：2013-03-01 00:00:00

abstract：:Participation in external quality assessment programs is critical to ensure quality clinical laboratory testing. Commercially available proficiency test panels for HIV-1 virus load testing that are used commonly in external quality assessment programs remain a financial obstacle to resource-limited countries. Maintain...

journal_title：Journal of virological methods

authors： Ramos A,Nguyen S,Garcia A,Subbarao S,Nkengasong JN,Ellenberger D

更新日期：2013-03-01 00:00:00

abstract：:Human papillomavirus (HPV) infection is the primary cause of cervical cancer. The Quantivirus(®) HPV E6/E7 RNA 3.0 assay (DiaCarta, CA, USA) detects E6/E7 mRNA of 13 high risk subtypes and 6 low risk subtypes. Cervical specimens collected in PreservCyt were processed for HPV detection. Cervical biopsies were taken onl...

journal_title：Journal of virological methods

authors： Shen Y,Gong J,He Y,Cheng G,Okunieff P,Li X

更新日期：2013-02-01 00:00:00

abstract：:HIV drug resistance (DR) testing using Sanger sequencing (SS) is limited by the inability of the method to identify low abundance drug resistance variants. The application of tagged pooled pyrosequencing (TPP) for HIV DR surveillance is described and the results compared with SS. HIV(+) serum specimens were genotyped ...

journal_title：Journal of virological methods

authors： Ji H,Liang B,Li Y,Van Domselaar G,Graham M,Tyler S,Merks H,Sandstrom P,Brooks J

更新日期：2013-02-01 00:00:00

abstract：:The development of an effective prophylactic vaccine is still necessary to improve the safety of the conventional although-discontinued smallpox vaccine, and to protect from the threat of deliberate release of variola virus. This need also arises from the number of new cases of animal orthopoxvirus infections each yea...

journal_title：Journal of virological methods

authors： Bissa M,Pacchioni SM,Zanotto C,De Giuli Morghen C,Radaelli A

更新日期：2013-01-01 00:00:00